Peripheral Blood MCEMP1 Gene Expression as a Biomarker for Stroke Prognosis
Background and Purpose—A limitation when making early decisions on stroke management is the lack of rapid diagnostic and prognostic testing. Our study sought to identify peripheral blood RNA biomarkers associated with stroke. The secondary aims were to assess the discriminative capacity of RNA bioma...
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Lopez-Jaramillo, Patricio Raman, Kripa O’Donnell, Martin J. Czlonkowska, Anna Duarte, Yan Carlos Peñaherrera, Ernesto Sharma, Mike Shoamanesh, Ashkan Skowronska, Marta Yusuf, Salim Paré, Guillaume 2019-07-02T12:33:44Z 2019-07-02T12:33:44Z 2016-01-12 7 p. Background and Purpose—A limitation when making early decisions on stroke management is the lack of rapid diagnostic and prognostic testing. Our study sought to identify peripheral blood RNA biomarkers associated with stroke. The secondary aims were to assess the discriminative capacity of RNA biomarkers for primary stroke type and stroke prognosis at 1-month. Methods—Whole-blood gene expression profiling was conducted on the discovery cohort: 129 first-time stroke cases that had blood sampling within 5 days of symptom onset and 170 control participants with no history of stroke. Results—Through multiple regression analysis, we determined that expression of the gene MCEMP1 had the strongest association with stroke of 11181 genes tested. MCEMP1 increased by 2.4-fold in stroke when compared with controls (95% confidence interval, 2.0–2.8; P=8.2×10−22). In addition, expression was elevated in intracerebral hemorrhage when compared with ischemic stroke cases (P=3.9×10−4). MCEMP1 was also highest soon after symptom onset and had no association with stroke risk factors. Furthermore, MCEMP1 expression independently improved discrimination of 1-month outcome. Indeed, discrimination models for disability and mortality that included MCEMP1 expression, baseline modified Rankin Scale score, and primary stroke type improved discrimination when compared with a model without MCEMP1 (disability Net Reclassification Index, 0.76; P=3.0×10−6 and mortality Net Reclassification Index, 1.3; P=1.1×10−9). Significant associations with MCEMP1 were confirmed in an independent validation cohort of 28 stroke cases and 34 controls. Conclusions—This study demonstrates that peripheral blood expression of MCEMP1 may have utility for stroke diagnosis and as a prognostic biomarker of stroke outcome at 1-month. application/pdf 10.1161/STROKEAHA.115.011854 1524-4628 0039-2499 https://repositorio.udes.edu.co/handle/001/3239 eng Stroke Derechos Reservados - American Heart Association, Inc., 2016 info:eu-repo/semantics/openAccess Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ https://www.ahajournals.org/doi/pdf/10.1161/STROKEAHA.115.011854 Biomarkers Blood Gene expression profiling Prognosis Stroke Peripheral Blood MCEMP1 Gene Expression as a Biomarker for Stroke Prognosis Artículo de revista http://purl.org/coar/resource_type/c_6501 Text info:eu-repo/semantics/article http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion Publication http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Peripheral Blood MCEMP1 Gene Expression as a Biomarker for Stroke Prognosis |
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Peripheral Blood MCEMP1 Gene Expression as a Biomarker for Stroke Prognosis Lopez-Jaramillo, Patricio Raman, Kripa O’Donnell, Martin J. Czlonkowska, Anna Duarte, Yan Carlos Peñaherrera, Ernesto Sharma, Mike Shoamanesh, Ashkan Skowronska, Marta Yusuf, Salim Paré, Guillaume Lopez-Jaramillo, Patricio Raman, Kripa O’Donnell, Martin J. Czlonkowska, Anna Duarte, Yan Carlos Peñaherrera, Ernesto Sharma, Mike Shoamanesh, Ashkan Skowronska, Marta Yusuf, Salim Paré, Guillaume Biomarkers Blood Gene expression profiling Prognosis Stroke |
title_short |
Peripheral Blood MCEMP1 Gene Expression as a Biomarker for Stroke Prognosis |
title_full |
Peripheral Blood MCEMP1 Gene Expression as a Biomarker for Stroke Prognosis |
title_fullStr |
Peripheral Blood MCEMP1 Gene Expression as a Biomarker for Stroke Prognosis |
title_full_unstemmed |
Peripheral Blood MCEMP1 Gene Expression as a Biomarker for Stroke Prognosis |
title_sort |
peripheral blood mcemp1 gene expression as a biomarker for stroke prognosis |
author |
Lopez-Jaramillo, Patricio Raman, Kripa O’Donnell, Martin J. Czlonkowska, Anna Duarte, Yan Carlos Peñaherrera, Ernesto Sharma, Mike Shoamanesh, Ashkan Skowronska, Marta Yusuf, Salim Paré, Guillaume Lopez-Jaramillo, Patricio Raman, Kripa O’Donnell, Martin J. Czlonkowska, Anna Duarte, Yan Carlos Peñaherrera, Ernesto Sharma, Mike Shoamanesh, Ashkan Skowronska, Marta Yusuf, Salim Paré, Guillaume |
author_facet |
Lopez-Jaramillo, Patricio Raman, Kripa O’Donnell, Martin J. Czlonkowska, Anna Duarte, Yan Carlos Peñaherrera, Ernesto Sharma, Mike Shoamanesh, Ashkan Skowronska, Marta Yusuf, Salim Paré, Guillaume Lopez-Jaramillo, Patricio Raman, Kripa O’Donnell, Martin J. Czlonkowska, Anna Duarte, Yan Carlos Peñaherrera, Ernesto Sharma, Mike Shoamanesh, Ashkan Skowronska, Marta Yusuf, Salim Paré, Guillaume |
building |
Repositorio digital |
topic |
Biomarkers Blood Gene expression profiling Prognosis Stroke |
topic_facet |
Biomarkers Blood Gene expression profiling Prognosis Stroke |
publishDate |
2016-01-12 |
language |
English |
format |
Artículo de revista |
description |
Background and Purpose—A limitation when making early decisions on stroke management is the lack of rapid diagnostic and prognostic testing. Our study sought to identify peripheral blood RNA biomarkers associated with stroke. The secondary aims were to assess the discriminative capacity of RNA biomarkers for primary stroke type and stroke prognosis at 1-month.
Methods—Whole-blood gene expression profiling was conducted on the discovery cohort: 129 first-time stroke cases that had blood sampling within 5 days of symptom onset and 170 control participants with no history of stroke. Results—Through multiple regression analysis, we determined that expression of the gene MCEMP1 had the strongest association with stroke of 11181 genes tested. MCEMP1 increased by 2.4-fold in stroke when compared with controls (95% confidence interval, 2.0–2.8; P=8.2×10−22). In addition, expression was elevated in intracerebral hemorrhage when compared with ischemic stroke cases (P=3.9×10−4). MCEMP1 was also highest soon after symptom onset and had no association with stroke risk factors. Furthermore, MCEMP1 expression independently improved discrimination of 1-month outcome. Indeed, discrimination models for disability and mortality that included MCEMP1 expression, baseline modified Rankin Scale score, and primary stroke type improved discrimination when compared with a model without MCEMP1 (disability Net Reclassification Index, 0.76; P=3.0×10−6 and mortality Net Reclassification Index, 1.3; P=1.1×10−9). Significant associations with MCEMP1 were confirmed in an independent validation cohort of 28 stroke cases and 34 controls.
Conclusions—This study demonstrates that peripheral blood expression of MCEMP1 may have utility for stroke
diagnosis and as a prognostic biomarker of stroke outcome at 1-month.
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issn |
0039-2499 |
url |
https://repositorio.udes.edu.co/handle/001/3239 |
url_str_mv |
https://repositorio.udes.edu.co/handle/001/3239 |
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1789502203253227520 |
score |
11.244619 |