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In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.

Background Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary. Objective To evaluate the antifung...

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Institution:Universidad EIA
Main Authors: Leal Pinto, Sandra-Milena, Herrera Sandoval, Laura-Viviana, Vargas Méndez, Leonor-Yamile, CLINIUDES
Format: Artículo de revista
Language:English
Published: Mycoses 2019-01
Edition:1 ed.
Subjects:
Cytotoxicity
Eugenol
Isoeugenol
Methylisoeugenol
Microsporum canis
Microsporum gypseum
Online Access:https://repositorio.udes.edu.co/handle/001/5657
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spelling Leal Pinto, Sandra-Milena
Herrera Sandoval, Laura-Viviana
Vargas Méndez, Leonor-Yamile
CLINIUDES
2021-10-04T16:03:51Z
2021-10-04T16:03:51Z
2019-01
Digital
Background Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary. Objective To evaluate the antifungal and cytotoxic potential of Eugenia caryophyllus essential oil (EO), eugenol, isoeugenol and methylisoeugenol against Microsporum canis, M. gypseum and Vero cells. Methods The EO was extracted by conventional heating-assisted hydrodistillation, the eugenol obtained commercially and the derivatives through Williamson synthesis. Minimal inhibitory concentration (MICs), minimum fungicidal concentration, inhibition of radial mycelial growth and germination inhibition were used to evaluate the antifungal activity. In addition, a colorimetric test was conducted to evaluate cytotoxic activity. Results MIC and MFC values for all compounds were 62.5-500 μg/mL for both of the species of Microsporum evaluated. Also, concentrations of 300 μg/mL of the compounds inhibited 100% of M. canis mycelium. The inhibition of germination was observed after 6 hours of treatment (11.86 ± 3.46-85.31 ± 0%). No cytotoxicity was observed in Vero cells (CC50 > 105 μg/mL), whereas terbinafine showed CC50 31.00 ± 0.61 μg/mL. Conclusions Our study indicates an interesting bioactivity of isoeugenol and methylisoeugenol against M. canis, M. gypseum and mammalian cells.
1 ed.
Ciencias básicas biomédicas, epidemiología, salud pública enfermedades infecciosas, enfermedades no transmisibles epidemiología, salud y desarrollo, discapacidad
1 p.
application/pdf
https://doi.org/10.1111/myc.12844
https://repositorio.udes.edu.co/handle/001/5657
eng
Mycoses
Reino Unido
50
1, enero de 2019
41
22
Leal Pinto, SM, Herrera Sandoval, LV, Vargas, LY. In vitro susceptibility of Microsporum spp. and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. Mycoses. 2019; 62: 41– 50. https://doi.org/10.1111/myc.12844
Mycoses, Volume62, Issue1 January 2019 Pages 41-50
Mycoses
The Editor(s) and the Author(s), 2019
info:eu-repo/semantics/openAccess
Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)
https://creativecommons.org/licenses/by-nc/4.0/
https://onlinelibrary.wiley.com/doi/full/10.1111/myc.12844
Cytotoxicity
Eugenol
Isoeugenol
Methylisoeugenol
Microsporum canis
Microsporum gypseum
In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
Artículo de revista
http://purl.org/coar/resource_type/c_6501
Text
info:eu-repo/semantics/article
http://purl.org/redcol/resource_type/ART
info:eu-repo/semantics/publishedVersion
Todas las Audiencias
Publication
http://purl.org/coar/access_right/c_f1cf
http://purl.org/coar/version/c_970fb48d4fbd8a85
institution Universidad EIA
collection d_repositorio.udes.edu.co-DSPACE
title In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
spellingShingle In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
Leal Pinto, Sandra-Milena
Herrera Sandoval, Laura-Viviana
Vargas Méndez, Leonor-Yamile
Leal Pinto, Sandra-Milena
Herrera Sandoval, Laura-Viviana
Vargas Méndez, Leonor-Yamile
CLINIUDES
Cytotoxicity
Eugenol
Isoeugenol
Methylisoeugenol
Microsporum canis
Microsporum gypseum
title_short In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
title_full In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
title_fullStr In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
title_full_unstemmed In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
title_sort in vitro susceptibility of microsporum spp and mammalian cells to eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
author Leal Pinto, Sandra-Milena
Herrera Sandoval, Laura-Viviana
Vargas Méndez, Leonor-Yamile
Leal Pinto, Sandra-Milena
Herrera Sandoval, Laura-Viviana
Vargas Méndez, Leonor-Yamile
CLINIUDES
author_facet Leal Pinto, Sandra-Milena
Herrera Sandoval, Laura-Viviana
Vargas Méndez, Leonor-Yamile
Leal Pinto, Sandra-Milena
Herrera Sandoval, Laura-Viviana
Vargas Méndez, Leonor-Yamile
CLINIUDES
building Repositorio digital
topic Cytotoxicity
Eugenol
Isoeugenol
Methylisoeugenol
Microsporum canis
Microsporum gypseum
topic_facet Cytotoxicity
Eugenol
Isoeugenol
Methylisoeugenol
Microsporum canis
Microsporum gypseum
publishDate 2019-01
language English
publisher Mycoses
physical 1 p.
format Artículo de revista
description Background Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary. Objective To evaluate the antifungal and cytotoxic potential of Eugenia caryophyllus essential oil (EO), eugenol, isoeugenol and methylisoeugenol against Microsporum canis, M. gypseum and Vero cells. Methods The EO was extracted by conventional heating-assisted hydrodistillation, the eugenol obtained commercially and the derivatives through Williamson synthesis. Minimal inhibitory concentration (MICs), minimum fungicidal concentration, inhibition of radial mycelial growth and germination inhibition were used to evaluate the antifungal activity. In addition, a colorimetric test was conducted to evaluate cytotoxic activity. Results MIC and MFC values for all compounds were 62.5-500 μg/mL for both of the species of Microsporum evaluated. Also, concentrations of 300 μg/mL of the compounds inhibited 100% of M. canis mycelium. The inhibition of germination was observed after 6 hours of treatment (11.86 ± 3.46-85.31 ± 0%). No cytotoxicity was observed in Vero cells (CC50 > 105 μg/mL), whereas terbinafine showed CC50 31.00 ± 0.61 μg/mL. Conclusions Our study indicates an interesting bioactivity of isoeugenol and methylisoeugenol against M. canis, M. gypseum and mammalian cells.
edition 1 ed.
url https://repositorio.udes.edu.co/handle/001/5657
url_str_mv https://repositorio.udes.edu.co/handle/001/5657
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score 11.341544