In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives.
Background Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary. Objective To evaluate the antifung...
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Mycoses
2019-01
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Leal Pinto, Sandra-Milena Herrera Sandoval, Laura-Viviana Vargas Méndez, Leonor-Yamile CLINIUDES 2021-10-04T16:03:51Z 2021-10-04T16:03:51Z 2019-01 Digital Background Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary. Objective To evaluate the antifungal and cytotoxic potential of Eugenia caryophyllus essential oil (EO), eugenol, isoeugenol and methylisoeugenol against Microsporum canis, M. gypseum and Vero cells. Methods The EO was extracted by conventional heating-assisted hydrodistillation, the eugenol obtained commercially and the derivatives through Williamson synthesis. Minimal inhibitory concentration (MICs), minimum fungicidal concentration, inhibition of radial mycelial growth and germination inhibition were used to evaluate the antifungal activity. In addition, a colorimetric test was conducted to evaluate cytotoxic activity. Results MIC and MFC values for all compounds were 62.5-500 μg/mL for both of the species of Microsporum evaluated. Also, concentrations of 300 μg/mL of the compounds inhibited 100% of M. canis mycelium. The inhibition of germination was observed after 6 hours of treatment (11.86 ± 3.46-85.31 ± 0%). No cytotoxicity was observed in Vero cells (CC50 > 105 μg/mL), whereas terbinafine showed CC50 31.00 ± 0.61 μg/mL. Conclusions Our study indicates an interesting bioactivity of isoeugenol and methylisoeugenol against M. canis, M. gypseum and mammalian cells. 1 ed. Ciencias básicas biomédicas, epidemiología, salud pública enfermedades infecciosas, enfermedades no transmisibles epidemiología, salud y desarrollo, discapacidad 1 p. application/pdf https://doi.org/10.1111/myc.12844 https://repositorio.udes.edu.co/handle/001/5657 eng Mycoses Reino Unido 50 1, enero de 2019 41 22 Leal Pinto, SM, Herrera Sandoval, LV, Vargas, LY. In vitro susceptibility of Microsporum spp. and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. Mycoses. 2019; 62: 41– 50. https://doi.org/10.1111/myc.12844 Mycoses, Volume62, Issue1 January 2019 Pages 41-50 Mycoses The Editor(s) and the Author(s), 2019 info:eu-repo/semantics/openAccess Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ https://onlinelibrary.wiley.com/doi/full/10.1111/myc.12844 Cytotoxicity Eugenol Isoeugenol Methylisoeugenol Microsporum canis Microsporum gypseum In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. Artículo de revista http://purl.org/coar/resource_type/c_6501 Text info:eu-repo/semantics/article http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion Todas las Audiencias Publication http://purl.org/coar/access_right/c_f1cf http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Universidad EIA |
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title |
In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. |
spellingShingle |
In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. Leal Pinto, Sandra-Milena Herrera Sandoval, Laura-Viviana Vargas Méndez, Leonor-Yamile Leal Pinto, Sandra-Milena Herrera Sandoval, Laura-Viviana Vargas Méndez, Leonor-Yamile CLINIUDES Cytotoxicity Eugenol Isoeugenol Methylisoeugenol Microsporum canis Microsporum gypseum |
title_short |
In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. |
title_full |
In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. |
title_fullStr |
In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. |
title_full_unstemmed |
In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. |
title_sort |
in vitro susceptibility of microsporum spp and mammalian cells to eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives. |
author |
Leal Pinto, Sandra-Milena Herrera Sandoval, Laura-Viviana Vargas Méndez, Leonor-Yamile Leal Pinto, Sandra-Milena Herrera Sandoval, Laura-Viviana Vargas Méndez, Leonor-Yamile CLINIUDES |
author_facet |
Leal Pinto, Sandra-Milena Herrera Sandoval, Laura-Viviana Vargas Méndez, Leonor-Yamile Leal Pinto, Sandra-Milena Herrera Sandoval, Laura-Viviana Vargas Méndez, Leonor-Yamile CLINIUDES |
building |
Repositorio digital |
topic |
Cytotoxicity Eugenol Isoeugenol Methylisoeugenol Microsporum canis Microsporum gypseum |
topic_facet |
Cytotoxicity Eugenol Isoeugenol Methylisoeugenol Microsporum canis Microsporum gypseum |
publishDate |
2019-01 |
language |
English |
publisher |
Mycoses |
physical |
1 p. |
format |
Artículo de revista |
description |
Background
Microsporum spp. are keratinophilic dermatophytes that mainly invade the stratum corneum of the skin and hair causing clinical symptoms associated with tinea. Its treatment has several limitations, and the search for new active molecules is necessary.
Objective
To evaluate the antifungal and cytotoxic potential of Eugenia caryophyllus essential oil (EO), eugenol, isoeugenol and methylisoeugenol against Microsporum canis, M. gypseum and Vero cells.
Methods
The EO was extracted by conventional heating-assisted hydrodistillation, the eugenol obtained commercially and the derivatives through Williamson synthesis. Minimal inhibitory concentration (MICs), minimum fungicidal concentration, inhibition of radial mycelial growth and germination inhibition were used to evaluate the antifungal activity. In addition, a colorimetric test was conducted to evaluate cytotoxic activity.
Results
MIC and MFC values for all compounds were 62.5-500 μg/mL for both of the species of Microsporum evaluated. Also, concentrations of 300 μg/mL of the compounds inhibited 100% of M. canis mycelium. The inhibition of germination was observed after 6 hours of treatment (11.86 ± 3.46-85.31 ± 0%). No cytotoxicity was observed in Vero cells (CC50 > 105 μg/mL), whereas terbinafine showed CC50 31.00 ± 0.61 μg/mL.
Conclusions
Our study indicates an interesting bioactivity of isoeugenol and methylisoeugenol against M. canis, M. gypseum and mammalian cells.
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edition |
1 ed. |
url |
https://repositorio.udes.edu.co/handle/001/5657 |
url_str_mv |
https://repositorio.udes.edu.co/handle/001/5657 |
_version_ |
1789502137888145408 |
score |
11.341544 |