Staff View: Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.

Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.

Identification of the bloodmeal sources of phlebotomine sand flies is fundamental to determining which species are anthropophilic and understanding the transmission of Leishmania parasites in natural epidemiological settings. The objective of this study was to identify sand fly bloodmeals in the mix...

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Institution:	Universidad de Sucre
Main Authors:	Paternina, Luís E., Verbel Vergara, Daniel, Romero Ricardo, Luís, Pérez Doria, Alveiro, Paternina Gómez, Margaret, Martínez, Lily, Bejarano, Eduar E.
Format:	Artículo de revista
Language:	English
Published:	Reino Unido: Acta Tropica, 2015. 2019-11-06
Subjects:	Lutzomyia Sandflies Bloodmeals Molecular analysis Leishmaniasis Colombia
Online Access:	https://repositorio.unisucre.edu.co/handle/001/956
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spelling	Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E. Colombia 2019-11-06T16:08:15Z 2019-11-06T16:08:15Z 2019-11-06 Paternina, L.E., Verbel, D., Romero, L., Pérez, A., Paternina, M., Martínez, L. & Bejarano, E.E. (2015). Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals. Acta Tropica. 153: 86 - 92. 0001-706X. https://repositorio.unisucre.edu.co/handle/001/956 http://dx.doi.org/doi:10.1016/j.actatropica.2015.10.005. Artículo digital. Identification of the bloodmeal sources of phlebotomine sand flies is fundamental to determining which species are anthropophilic and understanding the transmission of Leishmania parasites in natural epidemiological settings. The objective of this study was to identify sand fly bloodmeals in the mixed leishmaniasis focus of the department of Sucre, northern Colombia. In all 141 engorged female sand flies were analyzed, after being captured in intradomiciliary, peridomiciliary and extradomiciliary habitats with Shannon and CDC traps and by active searching in diurnal resting sites. Bloodmeals were identified by sequencing and analysis of a 358 bp fragment of the mitochondrial gene Cytochrome b (CYB) and a 330 bp fragment of the nuclear gene prepronociceptin (PNOC). Using both genes 105 vertebrate bloodmeals were identified, with an efficiency of 72% for CYB but only 7% for PNOC. Ten species of vertebrates were identified as providing bloodmeal sources for eight sand fly species: Homo sapiens (Lutzomyia evansi, Lu. panamensis, Lu. micropyga, Lu. shannoni and Lu. atroclavata), Equus caballus (Lu. evansi, Lu. panamensis and Lu. cayennensis cayennensis), Eq. asinus (Lu. evansi and Lu. panamensis), Bos taurus 4 (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Tamandua mexicana (Lu. shannoni and Lu. trinidadensis), Proechimys guyanensis (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Mabuya sp. (Lu. micropyga), Sus scrofa (Lu. evansi and Lu. gomezi) and Gallus gallus (Lu. evansi). Cattle, donkeys, humans and pigs were significantly more important than other animals (P = 0.0001) as hosts of Lu. evansi, this being the most abundant sand fly species. The five Lutzomyia species in which blood samples of human origin were detected included Lu. micropyga and Lu. atroclavata, constituting the first evidence of anthropophily in both species. application/pdf eng Reino Unido: Acta Tropica, 2015. Revista Alexander, B., 2000. Sampling methods for phlebotomine sand flies (Diptera: Psychodidae). Med. Vet. Entomol. 14, 1–13. Alexander, B., de Carvalho, R.L., McCallum, H., Pereira, M.H., 2002. Role of the domestic chicken (Gallus gallus) in the epidemiology of urban visceral leishmaniasis in Brazil. Emerg. Infect. Dis. 8, 1480–1485. Alexander, B., Lozano, C., Barker, D.C., McCann, S.H., Adler, G.H., 1998. Detection of Leishmania (Viannia) braziliensis complex in wild mammals from Colombian coffee plantations by PCR and DNA hybridization. Acta Trop. 69, 41–50. Alvar, J., Vélez, I.D., Bern, C., Herrero, M., Desjeux, P., Cano, J., Jannin, J., den Boer, M., 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS ONE 7, e35671. doi:10.1371/journal.pone.0035671. Bejarano E.E., Estrada L.G., 2015. Family Psychodidae, in: Wolff, M., Nihei, S.S., Pérez, S.P., & de Carvalho, C.J.B. (Eds.), Catalogue of Diptera of Colombia. Zootaxa, In Press. Derechos Reservados - Universidad de Sucre, 2019 https://creativecommons.org/licenses/by-nc/4.0/ info:eu-repo/semantics/openAccess Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://www.ncbi.nlm.nih.gov/pubmed/26464046. Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals. Artículo de revista info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 Text http://purl.org/coar/resource_type/c_2df8fbb1 Lutzomyia Sandflies Bloodmeals Molecular analysis Leishmaniasis Colombia http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/version/c_970fb48d4fbd8a85 Publication
institution	Universidad de Sucre
collection	d_repositorio.unisucre.edu.co-DSPACE
title	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
spellingShingle	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals. Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E. Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E. Lutzomyia Sandflies Bloodmeals Molecular analysis Leishmaniasis Colombia
title_short	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title_full	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title_fullStr	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title_full_unstemmed	Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.
title_sort	evidence for anthropophily in five species of phlebotomine sand flies (diptera: psychodidae) from northern colombia, revealed by molecular identification of bloodmeals.
author	Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E. Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E.
author_facet	Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E. Paternina, Luís E. Verbel Vergara, Daniel Romero Ricardo, Luís Pérez Doria, Alveiro Paternina Gómez, Margaret Martínez, Lily Bejarano, Eduar E.
building	Repositorio digital
topic	Lutzomyia Sandflies Bloodmeals Molecular analysis Leishmaniasis Colombia
topic_facet	Lutzomyia Sandflies Bloodmeals Molecular analysis Leishmaniasis Colombia
publishDate	2019-11-06
language	English
publisher	Reino Unido: Acta Tropica, 2015.
format	Artículo de revista
description	Identification of the bloodmeal sources of phlebotomine sand flies is fundamental to determining which species are anthropophilic and understanding the transmission of Leishmania parasites in natural epidemiological settings. The objective of this study was to identify sand fly bloodmeals in the mixed leishmaniasis focus of the department of Sucre, northern Colombia. In all 141 engorged female sand flies were analyzed, after being captured in intradomiciliary, peridomiciliary and extradomiciliary habitats with Shannon and CDC traps and by active searching in diurnal resting sites. Bloodmeals were identified by sequencing and analysis of a 358 bp fragment of the mitochondrial gene Cytochrome b (CYB) and a 330 bp fragment of the nuclear gene prepronociceptin (PNOC). Using both genes 105 vertebrate bloodmeals were identified, with an efficiency of 72% for CYB but only 7% for PNOC. Ten species of vertebrates were identified as providing bloodmeal sources for eight sand fly species: Homo sapiens (Lutzomyia evansi, Lu. panamensis, Lu. micropyga, Lu. shannoni and Lu. atroclavata), Equus caballus (Lu. evansi, Lu. panamensis and Lu. cayennensis cayennensis), Eq. asinus (Lu. evansi and Lu. panamensis), Bos taurus 4 (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Tamandua mexicana (Lu. shannoni and Lu. trinidadensis), Proechimys guyanensis (Lu. evansi, Lu. panamensis and Lu. c. cayennensis), Mabuya sp. (Lu. micropyga), Sus scrofa (Lu. evansi and Lu. gomezi) and Gallus gallus (Lu. evansi). Cattle, donkeys, humans and pigs were significantly more important than other animals (P = 0.0001) as hosts of Lu. evansi, this being the most abundant sand fly species. The five Lutzomyia species in which blood samples of human origin were detected included Lu. micropyga and Lu. atroclavata, constituting the first evidence of anthropophily in both species.
geographic_facet	Colombia
issn	0001-706X.
url	https://repositorio.unisucre.edu.co/handle/001/956
url_str_mv	https://repositorio.unisucre.edu.co/handle/001/956
_version_	1797111826783141888
score	11.246257

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Evidence for anthropophily in five species of phlebotomine sand flies (Diptera: Psychodidae) from northern Colombia, revealed by molecular identification of bloodmeals.