Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer
Background: Gastric Cancer is highly prevalent and deadly worldwide. In Colombia, it is the most lethal form of cancer. Some single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes have been associated with an anti-inflammatory environment and a Th2 profile in detriment of the antitumor T...
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Cárdenas, Denny Miley Sánchez, Angie Carolina Rosas, Daris Angélica Rivero López, Esmeralda Paparoni, Massiel Dayana Cruz, Mildred Andreína Suárez, Yeicy Paola Galvis Serrano, Nestor Fabián 2019-06-18T22:17:04Z 2019-06-18T22:17:04Z 2018-12-10 13 p. Background: Gastric Cancer is highly prevalent and deadly worldwide. In Colombia, it is the most lethal form of cancer. Some single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes have been associated with an anti-inflammatory environment and a Th2 profile in detriment of the antitumor Th1 response. This research sought to detect single-nucleotide polymorphisms in promoter sequences, like − 1082 (G/A), − 592 (C/A), and − 819 (C/T), as well as − 590 (C/T) of the IL-10 and IL-4 genes, respectively; in addition to the IL-4Rα mutation variants, Ile50Val and Q576R, together with circulating levels of IL-4, TNF-α, IL-10, and IFN-γ in patients with gastric carcinoma in Cúcuta, Colombia. Methods: In a cross-sectional study, 17 patients and 30 healthy individuals were genotyped for the six polymorphisms mentioned through PCR-RFLP of DNA obtained from peripheral blood cells and serum samples were analyzed by sandwich ELISA to quantify cytokines. Statistical difference between groups was determined along with the association between the presence of polymorphisms and the risk of gastric cancer, as well as the mortality in patients, using Mann-Whitney U test and logistic regression analysis, respectively. Results: An association between the − 1082 (G/A) and the risk of gastric cancer was found (OR = 7.58, range 0.77– 74.06, P = 0.08). Furthermore, patients had a significant increase in IL-4 serum levels (P < 0.01) compared to healthy individuals, both variables showed a higher estimated risk of mortality in patients, although without statistical association (P > 0.05). Conclusion: We infer that two possible biomarkers (one immunological and one genetic) could be considered in association with gastric cancer in our population, which should be confirmed by subsequent studies involving a greater number of individuals. application/pdf 10.1186/s12876-018-0913-9 1471-230X https://repositorio.udes.edu.co/handle/001/3121 eng BMC Gastroenterology Derechos Reservados - BioMed Central Ltd unless otherwise stated. Part of Springer Nature, 2018 info:eu-repo/semantics/openAccess Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0) https://creativecommons.org/licenses/by-nc/4.0/ https://bmcgastroenterol.biomedcentral.com/articles/10.1186/s12876-018-0913-9#Bib1 Genetic Polymorphism Cytokines Neoplasia Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer Artículo de revista http://purl.org/coar/resource_type/c_6501 Text info:eu-repo/semantics/article http://purl.org/redcol/resource_type/ART info:eu-repo/semantics/publishedVersion Publication http://purl.org/coar/access_right/c_abf2 http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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Universidad EIA |
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d_repositorio.udes.edu.co-DSPACE |
| title |
Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer |
| spellingShingle |
Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer Cárdenas, Denny Miley Sánchez, Angie Carolina Rosas, Daris Angélica Rivero López, Esmeralda Paparoni, Massiel Dayana Cruz, Mildred Andreína Suárez, Yeicy Paola Galvis Serrano, Nestor Fabián Cárdenas, Denny Miley Sánchez, Angie Carolina Rosas, Daris Angélica Rivero López, Esmeralda Paparoni, Massiel Dayana Cruz, Mildred Andreína Suárez, Yeicy Paola Galvis Serrano, Nestor Fabián Genetic Polymorphism Cytokines Neoplasia |
| title_short |
Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer |
| title_full |
Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer |
| title_fullStr |
Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer |
| title_full_unstemmed |
Preliminary analysis of single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes and profile of circulating cytokines in patients with gastric Cancer |
| title_sort |
preliminary analysis of single-nucleotide polymorphisms in il-10, il-4, and il-4rα genes and profile of circulating cytokines in patients with gastric cancer |
| author |
Cárdenas, Denny Miley Sánchez, Angie Carolina Rosas, Daris Angélica Rivero López, Esmeralda Paparoni, Massiel Dayana Cruz, Mildred Andreína Suárez, Yeicy Paola Galvis Serrano, Nestor Fabián Cárdenas, Denny Miley Sánchez, Angie Carolina Rosas, Daris Angélica Rivero López, Esmeralda Paparoni, Massiel Dayana Cruz, Mildred Andreína Suárez, Yeicy Paola Galvis Serrano, Nestor Fabián |
| author_facet |
Cárdenas, Denny Miley Sánchez, Angie Carolina Rosas, Daris Angélica Rivero López, Esmeralda Paparoni, Massiel Dayana Cruz, Mildred Andreína Suárez, Yeicy Paola Galvis Serrano, Nestor Fabián Cárdenas, Denny Miley Sánchez, Angie Carolina Rosas, Daris Angélica Rivero López, Esmeralda Paparoni, Massiel Dayana Cruz, Mildred Andreína Suárez, Yeicy Paola Galvis Serrano, Nestor Fabián |
| building |
Repositorio digital |
| topic |
Genetic Polymorphism Cytokines Neoplasia |
| topic_facet |
Genetic Polymorphism Cytokines Neoplasia |
| publishDate |
2018-12-10 |
| language |
English |
| format |
Artículo de revista |
| description |
Background: Gastric Cancer is highly prevalent and deadly worldwide. In Colombia, it is the most lethal form of
cancer. Some single-nucleotide polymorphisms in IL-10, IL-4, and IL-4Rα genes have been associated with an
anti-inflammatory environment and a Th2 profile in detriment of the antitumor Th1 response. This research sought
to detect single-nucleotide polymorphisms in promoter sequences, like − 1082 (G/A), − 592 (C/A), and − 819 (C/T),
as well as − 590 (C/T) of the IL-10 and IL-4 genes, respectively; in addition to the IL-4Rα mutation variants, Ile50Val
and Q576R, together with circulating levels of IL-4, TNF-α, IL-10, and IFN-γ in patients with gastric carcinoma in
Cúcuta, Colombia.
Methods: In a cross-sectional study, 17 patients and 30 healthy individuals were genotyped for the six
polymorphisms mentioned through PCR-RFLP of DNA obtained from peripheral blood cells and serum samples
were analyzed by sandwich ELISA to quantify cytokines. Statistical difference between groups was determined
along with the association between the presence of polymorphisms and the risk of gastric cancer, as well as the
mortality in patients, using Mann-Whitney U test and logistic regression analysis, respectively.
Results: An association between the − 1082 (G/A) and the risk of gastric cancer was found (OR = 7.58, range 0.77–
74.06, P = 0.08). Furthermore, patients had a significant increase in IL-4 serum levels (P < 0.01) compared to healthy
individuals, both variables showed a higher estimated risk of mortality in patients, although without statistical
association (P > 0.05).
Conclusion: We infer that two possible biomarkers (one immunological and one genetic) could be considered in
association with gastric cancer in our population, which should be confirmed by subsequent studies involving a
greater number of individuals.
|
| issn |
1471-230X |
| url |
https://repositorio.udes.edu.co/handle/001/3121 |
| url_str_mv |
https://repositorio.udes.edu.co/handle/001/3121 |
| _version_ |
1763049438777966592 |
| score |
11.257267 |
