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Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.

Disruption of vacuolar biogenesis in the pathogenic yeast Candida albicans causes profound defects in polarized hyphal growth. However, the precise vacuolar pathways involved in yeast-hypha differentiation have not been determined. Previously we focused on Vps21p, a Rab GTPase involved in directing...

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Institution:Universidad de Sucre
Main Authors: Johnston, Douglas A., Luna Tapia, Arturo, Eberle, Karen E., Palmer, Glen E., American society for microbiology.
Format: Artículo de revista
Language:English
Published: Estados Unidos: Eukaryotic Cell , 2013. 2019-10-03
Subjects:
Candida albicans
Neoformans
vacuolar
Online Access:https://repositorio.unisucre.edu.co/handle/001/835
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id oai:repositorio.unisucre.edu.co:001-835
recordtype dspace
spelling Johnston, Douglas A.
Luna Tapia, Arturo
Eberle, Karen E.
Palmer, Glen E.
American society for microbiology.
Colombia
2019-10-03T21:59:59Z
2019-10-03T21:59:59Z
2019-10-03
Johnton, D.A., Luna, A., Eberle, K.E. & Palmer, G.E. (2013). Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth. Eukaryotic Cell. 12 (7), 1039 –1050.
1535-9786
https://repositorio.unisucre.edu.co/handle/001/835
10.1128/EC.00359-12
Artículo digital.
Disruption of vacuolar biogenesis in the pathogenic yeast Candida albicans causes profound defects in polarized hyphal growth. However, the precise vacuolar pathways involved in yeast-hypha differentiation have not been determined. Previously we focused on Vps21p, a Rab GTPase involved in directing vacuolar trafficking through the late endosomal prevacuolar compartment (PVC). Herein, we identify two additional Vps21p-related GTPases, Ypt52p and Ypt53p, that colocalize with Vps21p and can suppress the hyphal defects of the vps21 / mutant. Phenotypic analysis of gene deletion strains revealed that loss of both VPS21 and YPT52 causes synthetic defects in endocytic trafficking to the vacuole, as well as delivery of the virulence-associated vacuolar membrane protein Mlt1p from the Golgi compartment. Transcription of all three GTPase-encoding genes is increased under hyphal growth conditions, and overexpression of the transcription factor Ume6p is sufficient to increase the transcription of these genes. While only the vps21 / single mutant has hyphal growth defects, these were greatly exacerbated in a vps21 / ypt52 / double mutant. On the basis of relative expression levels and phenotypic analysis of gene deletion strains, Vps21p is the most important of the three GTPases, followed by Ypt52p, while Ypt53p has an only marginal impact on C. albicans physiology. Finally, disruption of a nonendosomal AP-3-dependent vacuolar trafficking pathway in the vps21 / ypt52 / mutant, further exacerbated the stress and hyphal growth defects. These findings underscore the importance of membrane trafficking through the PVC in sustaining the invasive hyphal growth form of C. albicans.
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Estados Unidos: Eukaryotic Cell , 2013.
Revista
Hu G, Hacham M, Waterman SR, Panepinto J, Shin S, Liu X, Gibbons J, Valyi-Nagy T, Obara K, Jaffe HA, Ohsumi Y, Williamson PR. 2008. PI3K signaling of autophagy is required for starvation tolerance and virulence of Cryptococcus neoformans. J. Clin. Invest. 118:1186 –1197.
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Palmer GE. 2010. Endosomal and AP-3-dependent vacuolar trafficking routes make additive contributions to Candida albicans hyphal growth and pathogenesis. Eukaryot. Cell 9:1755–1765.
4. Hilty J, Smulian AG, Newman SL. 2008. The Histoplasma capsulatum vacuolar ATPase is required for iron homeostasis, intracellular replication in macrophages and virulence in a murine model of histoplasmosis. Mol. Microbiol. 70:127–139.
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Derechos Reservados - Universidad de Sucre, 2019
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info:eu-repo/semantics/openAccess
Atribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)
https://ec.asm.org/content/12/7/1039.
Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.
Artículo de revista
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
Text
http://purl.org/coar/resource_type/c_2df8fbb1
Candida albicans
Neoformans
vacuolar
http://purl.org/coar/access_right/c_abf2
http://purl.org/coar/version/c_970fb48d4fbd8a85
institution Universidad de Sucre
collection d_repositorio.unisucre.edu.co-DSPACE
title Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.
spellingShingle Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.
Johnston, Douglas A.
Luna Tapia, Arturo
Eberle, Karen E.
Palmer, Glen E.
Johnston, Douglas A.
Luna Tapia, Arturo
Eberle, Karen E.
Palmer, Glen E.
American society for microbiology.
Candida albicans
Neoformans
vacuolar
title_short Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.
title_full Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.
title_fullStr Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.
title_full_unstemmed Three prevacuolar compartment Rab GTPases impact candida albicans hyphal growth.
title_sort three prevacuolar compartment rab gtpases impact candida albicans hyphal growth.
author Johnston, Douglas A.
Luna Tapia, Arturo
Eberle, Karen E.
Palmer, Glen E.
Johnston, Douglas A.
Luna Tapia, Arturo
Eberle, Karen E.
Palmer, Glen E.
American society for microbiology.
author_facet Johnston, Douglas A.
Luna Tapia, Arturo
Eberle, Karen E.
Palmer, Glen E.
Johnston, Douglas A.
Luna Tapia, Arturo
Eberle, Karen E.
Palmer, Glen E.
American society for microbiology.
building Repositorio digital
topic Candida albicans
Neoformans
vacuolar
topic_facet Candida albicans
Neoformans
vacuolar
publishDate 2019-10-03
language English
publisher Estados Unidos: Eukaryotic Cell , 2013.
format Artículo de revista
description Disruption of vacuolar biogenesis in the pathogenic yeast Candida albicans causes profound defects in polarized hyphal growth. However, the precise vacuolar pathways involved in yeast-hypha differentiation have not been determined. Previously we focused on Vps21p, a Rab GTPase involved in directing vacuolar trafficking through the late endosomal prevacuolar compartment (PVC). Herein, we identify two additional Vps21p-related GTPases, Ypt52p and Ypt53p, that colocalize with Vps21p and can suppress the hyphal defects of the vps21 / mutant. Phenotypic analysis of gene deletion strains revealed that loss of both VPS21 and YPT52 causes synthetic defects in endocytic trafficking to the vacuole, as well as delivery of the virulence-associated vacuolar membrane protein Mlt1p from the Golgi compartment. Transcription of all three GTPase-encoding genes is increased under hyphal growth conditions, and overexpression of the transcription factor Ume6p is sufficient to increase the transcription of these genes. While only the vps21 / single mutant has hyphal growth defects, these were greatly exacerbated in a vps21 / ypt52 / double mutant. On the basis of relative expression levels and phenotypic analysis of gene deletion strains, Vps21p is the most important of the three GTPases, followed by Ypt52p, while Ypt53p has an only marginal impact on C. albicans physiology. Finally, disruption of a nonendosomal AP-3-dependent vacuolar trafficking pathway in the vps21 / ypt52 / mutant, further exacerbated the stress and hyphal growth defects. These findings underscore the importance of membrane trafficking through the PVC in sustaining the invasive hyphal growth form of C. albicans.
geographic_facet Colombia
issn 1535-9786
url https://repositorio.unisucre.edu.co/handle/001/835
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